just done watching Smile (a drama starring Matsumoto Jun) and it was good. The ending was satisfying... ^^

Looking for a another nice drama...

1. Add in 1 mL of 2000 Dextran blue.

2. Let it flow through the bed until all the Dextran blue was within the chromatographic bead before adding in dH2O. This is to prevent dilution of the Dextran blue

3. let it flow until the blue color reaches the bottom of the column.

4. The volume collected were measured with a cylinder to obtain the void volume.

1. weigh 3g of chromatography beads (for 50 mL of column) --> this depends on the size of the column. the beads usually swell 5-6 times its original size after heating.

2. Add in distilled water and starts stirring it with magnetic stirrer and heating it at 60 celcius. Discard the impurities on the upper layer once in a while until the mixture was free of impurities.

3. Incubate in the oven overnight for full swelling.

4. The bottom of the column was stuffed with cotton.

5. The swollen beads was poured into the column slowly.

6. The screw of the cork at the bottom of the column was opened to release water (slowly for so that the beads would pack better)

7. When the column was more than half packed, the screw of the cork was opened to its maximum.

8. While packing the wall of the column should be knock gently to allow uniform packing.

1. Chromatography beads are very expensive. It is recyclable. Even if the column cracked during packing, it could still be repacked. Never discard it.

2. Do not start doing things without consulting your senior

3. Chromatography beads have to be kept in the fridge (4 celcius) after recollecting it. It should be immersed in distilled water(or its buffer).

4. Proteins: if are to be left for more than a day has to be kept in the fridge.

5. Tissue papers, gloves, and noodle cups go into the waste paper basket and not the biohazard plastic bag.

6. After centrifugation, pour the supernatant in one go.

a net friend of mine linked me to one of her favourite videos --> http://www.youtube.com/watch?v=dHEtZNI4Ynw
I watched it and fell in love with the background song... The song is by Kanjani8 --> B.J. from their 8th single "Wahaha"

I found the lyrics of this song too, with english translation...
http://goro-chan.livejournal.com/103893.html?view=292821#t292821
Thanks, Goro-chan for taking the time to translate it...

I'm just too pissed off with people who are still distributing fansubs although they had promised not to do so when they join the community!!! They're making everyone's life harder and harder. Dramacrazy, huh? stealing other people's hard work and they still have the nerve to mock the fansubbers! ArrgGGHhhh~~!! DAMN IT!

I had my panda eyes on today because i cried a lot yesterday... I happened to watch a raw video of Tensai Shimura Doubutsuen (2008.04.12) last night. Only then that i realize that the movie of "A tale of Mari and Three Puppies" was based on a true story. I searched high and low for the download links / torrent for the movie but failed. Instead i found a blog (http://redstarcafe.wordpress.com/2008/01/26/mari-and-the-niigata-earthquake/) which gives a complete summary of the movie. Read it, watched the trailer and cried again.

MySpace Playlist at MixPod.com

Found this today from wikihow.com:


How to Control Your Temper

1. Remember that the reward for patience is happiness.
2. Realize that things can be handled well at all times; it's just a matter of what kind of attitude you put into it. Take deep breaths.
3. Get away from situations where your anger might get the better of you, such as when someone is teasing you. Ignore the other person and walk away. If you must, do this a few times until the person gets the hint that you will not tolerate it. It's okay to use your words or body language to tell somebody you will not tolerate how they are treating you, as long as you respond to them in a calm and matter of fact manner, without being overtly hurtful or trying to embarrass them as they have you. It's never okay to hit somebody for making fun.
4. Realize that no one likes to be around people who get angry easily, and act abusive. The only way you can change the situation is by starting with yourself.
5. Be considerate of others, and ultimately, yourself. The way you react will ultimately affect your surroundings. If you have to rebuke someone for doing something wrong, you can always do it calmly, in a private place. This method is always better for everyone in the long run.
6. If you are angry and you are standing, sit down, if you are sitting then lie down.
7. Remember, 5 seconds stand between you and logic, count to 5, and remember that logic always beats anger.
8. Stop talking to that person for few minutes to whom you are angry and take deep breath around 5-7 times.
9. (IF there are any) Think about the good things that person has done for you. What bad have you done to them? Chances are, you've done at least something to them to make them resentful towards you. Make sure you have a clear conscience-then speak.
10. Temper can ruin anybody's life. When your eyes are opened it might be too late, so try to control your anger and follow the above steps. It will definitely get you away from tension.
11. Count from 1 to 10 in ascending order and then descending order, you will feel a bit calmer.
12. Saying hurtful things could be very risky and dangerous and it can hurt someone's heart so please stay calm when you feel hyper.


And i think this is very useful especially for someone like me.... I really find it difficult to control my temper... Sometimes, because of my temper, i'm driving people away... T___T
I get angry very easily but i would regret very soon too... this is bad....

This post had been inspired by Angela (http://arashitonews.livejournal.com/).... - Sorry for stealing your idea.... >_<
I would really like to thank all the subbers and translators of Arashi videos for all the hard work... Without you, we'll have to watch the raw videos without understanding the contents... It is because you guys took and sacrifice your free time (or maybe chip in some time from your tight schedule) to work on the projects.... Again... thank you so much for all the hard work!!! You guys rock!!

For all the raw providers.... thank you so much for willing to share Arashi goodies with us.... Without you, we won't be able to update ourselves about Arashi... !!!! You guys rock!!

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Initials....


1. What is your name: Hee

2. A four-letter word: Hate

3. A boy's name: Harry

4. A girl's name: Hege

5. An occupation: Hygienist

6. A colour: Hotpink

7. Something you wear: Hot pants

8. A food : Hotdog

9. Something found in the bathroom: Hanger

10. A place: Hungary

11. A reason for being late: Hangover

12. Something you shout: HUH!?

13. A movie title: Hulk

14. Something you drink: Hot Chocolate

15. A musical group: Hey! Say! Jump

16. An animal: Hippo

17. A street name: High Groove Manor

18.The title of a song: Hit N Run

19. A type of car: Honda Accord

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