1. weigh 3g of chromatography beads (for 50 mL of column) --> this depends on the size of the column. the beads usually swell 5-6 times its original size after heating.

2. Add in distilled water and starts stirring it with magnetic stirrer and heating it at 60 celcius. Discard the impurities on the upper layer once in a while until the mixture was free of impurities.

3. Incubate in the oven overnight for full swelling.

4. The bottom of the column was stuffed with cotton.

5. The swollen beads was poured into the column slowly.

6. The screw of the cork at the bottom of the column was opened to release water (slowly for so that the beads would pack better)

7. When the column was more than half packed, the screw of the cork was opened to its maximum.

8. While packing the wall of the column should be knock gently to allow uniform packing.